Types of Biospecimens
The following biological specimens are used as biological samples from patients suffering from various diseases, as well as normal tissues and fluids:
• Tissues that have undergone processing
STANDARDS FOR FIXING AND SAVING BIOLOGICAL SAMPLES:
FFPE. FORMAL FIXING OF TISSUE SAMPLES AND PREPARATION OF PARAFFIN BLOCKS
A human tissue sample prepared by a certified pathologist is immersed and aged in 10% neutral formalin for 18 to 24 hours, after which it is stored in the paraffin block. Typically, the block size is 0.5 x 1 x 1 cm, but can vary significantly depending on the nature of the disease and the type of tissue. More information is available upon request.
FF. SHOCK FREEZING OF BIOLOGICAL TISSUES
Small pieces of tissue prepared by a certified pathologist are shock-frozen in liquid nitrogen, usually within 20 to 30 minutes after surgical excision or 4 to 12 hours after death in autopsy cases. The standard sample has an average weight of 0.5 – 2 g and is supplied in standard cryovial. More information is available upon request.
CELL AND TISSUE CULTURES
Central National Biobank LLC. applies effective protocols for the preparation and establishment of primary human cell lines (solid tumors, blood cancer, bone marrow cancer and normal tissues). Preparation of primary cell cultures provide maintenance of their differentiated state and can be used to study the corresponding changes in the disease and predict the effectiveness of the effects of new drug candidates. Using standard protocols, our employees will be able to prepare cell lines from most tissues of research interest to our partners. If you want to implement a specific project, please contact us to discuss your wishes.
ISOLATION OF DNA AND RNA
Our standard protocols for the isolation of RNA and DNA from various types of biomaterials (tissues, paraffin blocks, body fluids and cell cultures) meet the quality requirements for most research methods, including NGS and others.
SEPARATION OF CELLULAR FRACTIONS
• Isolation of blood mononuclear cells (PBMC, peripheral blood mononuclear cells)
• Isolation of cell fractions from bone marrow (BMMC, bone marrow mononuclear cells)